As research continues to unravel the molecular basis of regulatory T cells (Treg), FOXP3 has emerged as a key player in orchestrating development and function of natural Treg. Two recent publications identified GPR83 as specifically upregulated in fresh and activated CD4+ CD25+ Treg cells. Both groups performed gene expression profiling on Treg, and other T cells to identify differentially regulated genes. Sugimoto et al. performed additional mRNA and protein expression analysis based on the results of their micro array analysis using RT-PCR as well as FACS analysis of GPR83 using anti-GPR83 antibody. These recent findings suggest that GPR83 may be useful as a specific and stable cell surface marker with modulatory properties, and a key molecule for further characterizing the molecular basis of Tregs. IMGENEX proudly offers this Flow and IHC positive GPR83 antibody (IMG-71561), as well as other GPR83, FOXP3, and CD marker antibodies for your Treg research.

Key Publication Findings

?GPR83 is upregulated in both murine and human Treg ? Gene analysis, mRNA, and protein expression show that GPR83 is upregulated in FOXP3+ cells

? GPR83 is expressed in both fresh and activated Treg

? GPR83 appears to be FOXP3 dependent, although Hansen et al. suggests that induction of FOXP3+

Treg by GPR83 occurs in vivo, suggesting a more complex relationship

? GPR83 is found in both Thymus and Spleen/LN CD4 and CD25 cells

? Foxp3 retroviral transduced CD25- CD4+ cells induces GPR83 expression at much higher levels than mock-transduced cells

? Suggests a possible ligand that binds to GPR83 in vivo which confers to these cells suppressive activity.